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Rinse pouch under tap water. Place pouch in path of water flow in the aquarium filter. For a new aquarium or when ammonia or nitrite is detected: recharge every 5 days for the first month, and then every 2 weeks for the second month. For an established aquarium: recharge every 2 weeks. When nitrate is detected: recharge every 5 days until nitrate is reduced to 20 ppm (mg/L) or less. Replace pouch after 2 months.